Differential-Mobility Spectrometry of 1-Deoxysphingosine Isomers: New Insights into the Gas Phase Structures of Ionized Lipids

BLJ Poad; AT MacCarone; H Yu; TW Mitchell; EM Saied; C Arenz; T Hornemann; JN Bull; EJ Bieske; SJ Blanksby

Journal article

Differential-Mobility Spectrometry of 1-Deoxysphingosine Isomers: New Insights into the Gas Phase Structures of Ionized Lipids

BLJ Poad, AT MacCarone, H Yu, TW Mitchell, EM Saied, C Arenz, T Hornemann, JN Bull, EJ Bieske, SJ Blanksby

Analytical Chemistry | AMER CHEMICAL SOC | Published : 2018

DOI: 10.1021/acs.analchem.8b00469

Abstract

Separation and structural identification of lipids remain a major challenge for contemporary lipidomics. Regioisomeric lipids differing only in position(s) of unsaturation are often not differentiated by conventional liquid chromatography-mass spectrometry approaches leading to the incomplete, or sometimes incorrect, assignation of molecular structure. Here we describe an investigation of the gas phase separations by differential-mobility spectrometry (DMS) of a series of synthetic analogues of the recently described 1-deoxysphingosine. The dependence of the DMS behavior on the position of the carbon-carbon double bond within the ionized lipid is systematically explored and compared to trend..

View full abstract

University of Melbourne Researchers

Evan Bieske Author

Related Projects (2)

Ions in the Fire: Laser Spectroscopy of Cryogenically Cooled Molecular Ions

This project will combine sophisticated laser and mass spectrometric techniques to probe the structure and function of cryogenically cooled ..

Shape-Shifting Molecules: Photoisomerization Action Spectroscopy

This project aims to examine molecules that change shape in response to light in order to gain insight into the biological processes they co..

Grants

Awarded by National Computational Infrastructure

Funding Acknowledgements

This work was supported through funding from the Australian Research Council (ARC) Discovery Program (Grants DP140101237, DP150101715, DP150101427, and DP160100474) and through the ARC Linkage Scheme (Grant LP110200648 with SCIEX). Some of the results reported in this manuscript were obtained in the Central Analytical Research Facility (CARP), operated by the Institute for Future Environments (QUT). Access to CARF is supported by funding from the Science and Engineering Faculty (QUT). This project was undertaken with the assistance of resources and services from the National Computational Infrastructure (NCI), which is supported by the Australian Government. SJ.B and B.L.J.P thank Dr. Erin S. Baker for helpful discussions.